Coding

Part:BBa_K368009:Design

Designed by: Jara Radeck   Group: iGEM10_LMU-Munich   (2010-10-24)


tet-on Promoter+TEV recogn.site+N-degron+SF3b155+Human Bak+SV40PA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1
    Illegal XhoI site found at 2480
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 2029
    Illegal SapI.rc site found at 1201


Design Notes

-> "Cut'N'survive" system


Source

(Knop et al.: Efficient protein depletion by genetically controlled deprotection of a dormant N-degron (2009))


References